Uromodulin antibody

Catalog No. ABIN264838

This Sheep Polyclonal antibody specifically detects Uromodulin in ID. It exhibits reactivity toward Human.

Quick Overview for Uromodulin antibody (ABIN264838)

Target: Uromodulin (UMOD)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Uromodulin antibody is un-conjugated

Application: Immunodiffusion (ID)

Product Details anti-Uromodulin Antibody

Specificity: This antibody gives a single arc when tested by IEP against fresh urine. No arcs are visible when tested by IEP against serum. Identity has been confirmed by double diffusion (Ouchterlony) against uromucoid and a known anti-uromucoid.

Purification: Ion exchange chromatography

Immunogen: Human Uromucoid, purified from human urine

Application Details

Application Notes: The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. RID: 10l antiserum/cm2 in gel vs 5l uromucoid - 50 mg/L Neat - 1: 5. Double Diffusion: 10l antiserum vs 3l uromucoid - 50 mg/LIEP: 100l antiserum vs 5 μL uromucoid - 50 mg/L
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 14.8mg/mL (OD280nm E1% = 14.5)

Buffer: Glycine buffered saline, pH 7.4 containing 0.09 % Sodium azide, 0.1 % E-Amino-n-Caproic acid, 0.01 % Benzamidine, 1 mM EDTA

Preservative: Sodium azide, Benzamidine, EACA

Precaution of Use: This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Uromodulin

Target: Uromodulin (UMOD)

Background: Synonyms: THP, Tamm-Horsfall urinary glycoprotein

Gene ID: 7369

NCBI Accession: NP_001008390

UniProt: P07911