SOD2 antibody

Catalog No. ABIN264858

This anti-SOD2 antibody is a Rabbit Polyclonal antibody detecting SOD2 in WB, IP, IF, IHC (p), IHC (fro) and EIA. Suitable for Human, Rat, Mouse, Pig, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Hamster, Chicken, Xenopus laevis and Drosophila melanogaster.

Quick Overview for SOD2 antibody (ABIN264858)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Rat, Mouse, Pig, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Hamster, Chicken, Xenopus laevis, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-SOD2 Antibody

Specificity: Detects 25 kDa protein, corresponding to the molecular mass of Mn Superoxide Dismutase (SOD) on SDS-PAGE Immunoblots.

Purification: Antigen Affinity Chromatography

Immunogen: Rat Mn SOD

Isotype: IgG

Application Details

Application Notes: EIA (6). Immunoprecipitation (4). Immunohistochemistry on Frozen Sections (8). Western blot (6): 0.5 μg/mL was sufficient for detection of Mn SOD in 20 μg of Rat braintissue extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS ( pH 7.0), 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: Superoxide Dismutase 2 / SOD2

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDa and it exists only in the extracellular space(7). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: Superoxide dismutase Mn

Gene ID: 6648

NCBI Accession: NP_000627

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling