Acetylated Lysine antibody (acetylated)

Catalog No. ABIN264880

This Rabbit Polyclonal antibody specifically detects Acetylated Lysine in WB, IP, IF and EIA. It exhibits reactivity toward .

Quick Overview for Acetylated Lysine antibody (acetylated) (ABIN264880)

Target: Acetylated Lysine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Acetylated Lysine Antibody

Binding Specificity: acetylated

Purification: Affinity Chromatography

Immunogen: Acetylated KLH Conjugated

Isotype: IgG

Application Details

Application Notes: Western blot (6). Immunoprecipitation (7). ELISA. Immonoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Target Type: Chemical

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N?-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).Synonyms: AcK, acetyl Lysine, acetyl-Lysine