ACP1 antibody

Catalog No. ABIN264957

This anti-ACP1 antibody is a Sheep Polyclonal antibody detecting ACP1 in WB and IP. Suitable for Human, Rat, Mouse and Cow.

Quick Overview for ACP1 antibody (ABIN264957)

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Reactivity: Human, Rat, Mouse, Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This ACP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ACP1 Antibody

Specificity: This antibody reacts to LMW Protein Tyrosine Phosphatase.

Purification: Purified

Immunogen: The human protein, HCPTPA was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.

Isotype: IgG

Application Details

Application Notes: Western Blot. Immunoprecipitation: 1 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for ACP1

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Alternative Name: ACP1 / LMW-PTPase

Background: Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1) 2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.Synonyms: Adipocyte acid phosphatase, HAAP, Low molecular weight cytosolic acid phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Red cell acid phosphatase 1

Gene ID: 52

NCBI Accession: NP_004291

UniProt: P24666