RNF216 antibody (Isoform 1, N-Term)

Catalog No. ABIN265155

This anti-RNF216 antibody is a Rabbit Polyclonal antibody detecting RNF216 in WB. Suitable for Mouse.

Quick Overview for RNF216 antibody (Isoform 1, N-Term) (ABIN265155)

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF216 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF216 Antibody

Binding Specificity: Isoform 1, N-Term

Specificity: This antibody detects TRIAD3A. Located in the cytoplasm, TRIAD3A is ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes (Ref.6).

Purification: Purified

Immunogen: Peptide corresponding to 15 amino acids near the amino-terminus of Mouse TRIAD3A

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5-1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.

Target Details for RNF216

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Alternative Name: RNF216

Background: Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2). Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR (3,4). Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase (5). TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9, this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation (5).Synonyms: E3 ubiquitin-protein ligase RNF216, RING finger protein 216, TRIAD3, Triad domain-containing protein 3, UBCE7IP1, Ubiquitin-conjugating enzyme 7-interacting protein 1, ZIN, Zinc finger protein inhibiting NF-kappa-B

Gene ID: 54476

NCBI Accession: NP_996994

UniProt: Q9NWF9