SPARC antibody

Catalog No. ABIN265179

This anti-SPARC antibody is a Rabbit Polyclonal antibody detecting SPARC in WB, IHC (fro) and EIA. Suitable for Human and Mouse.

Quick Overview for SPARC antibody (ABIN265179)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-SPARC Antibody

Specificity: This antibody detects Human SPARC. May cross react with SPARCL1.

Purification: Antigen Immunoaffinity Chromatography

Immunogen: Synthetic peptide derived from the Human SPARC protein

Application Details

Application Notes: ELISA. Western Blot: 0.5-1 μg/mL. Positive control: mouse heart lysate. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.274 mg/mL

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon arrival freeze at -20 °C to -70 °C.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC / Osteonectin

Background: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy