SPARC antibody

Catalog No. ABIN265180

This Rabbit Polyclonal antibody specifically detects SPARC in WB, IHC (fro) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for SPARC antibody (ABIN265180)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-SPARC Antibody

Specificity: This antibody detects SPARC. It may Cross react with SPARCL1.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the Human SPARC protein

Isotype: IgG

Application Details

Application Notes: Western blot: (1-5 μg/mL). ELISA: Immunohistochemistry on Frozen Sections. Positive Control/Tissue Expression: Mouse heart lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC / Osteonectin

Background: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy