SET/TAF-I antibody

Catalog No. ABIN265242

The Rabbit Polyclonal anti-SET/TAF-I antibody (ABIN265242) specifically detects SET/TAF-I in WB. The antibody is reactive with Human samples.

Quick Overview for SET/TAF-I antibody (ABIN265242)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SET/TAF-I Antibody

Specificity: This antibody detects I2PP2A / SET.

Purification: Purified

Immunogen: Synthetic peptide derived from the Human SET protein

Isotype: IgG

Application Details

Application Notes: Western blot (1-5 μg/mL). Positive Control/Tissue Expression: widely expressed. Low levels in quiescent cells duringserum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET / I2PP2A

Background: Human SET was originally identified as part of the SET-CAN fusion gene produced by a somatic translocation event in a patient with acute undifferentiated leukemia. In developing kidney, SET is highly expressed in the zone of nephron morphogenesis. SET has been shown to be a potent and specific inhibitor of protein phosphatase 2A, a family of major serine/threonine phosphatases involved in regulating cell proliferation and differentiation. SET is also involved in the regulation of renal cell proliferation and tumorigenesis. SET mRNA expression is markedly reduced in cells rendered quiescent by serum starvation, contact inhibition, or differentiation. SET protein expression is also much greater in developing rat and human kidney than in fully differentiated, mature kidney. High levels of SET mRNA and SET protein expression arefound in Wilms' tumor, but not in renal cell carcinoma, adult polycystic kidney disease or in transitional cell carcinoma.Synonyms: I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I

Gene ID: 6418

NCBI Accession: NP_001116293

UniProt: H0UI37

Pathways: Apoptosis