SRPRB antibody (AA 246-265)

Catalog No. ABIN265297

This Sheep Polyclonal antibody specifically detects SRPRB in WB and IHC (fro). It exhibits reactivity toward Dog and Chicken.

Quick Overview for SRPRB antibody (AA 246-265) (ABIN265297)

Target: SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))

Reactivity: Dog, Chicken

Host: Sheep

Clonality: Polyclonal

Conjugate: This SRPRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SRPRB Antibody

Binding Specificity: AA 246-265

Specificity: This antibody detects SRPR beta.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 246-265 derived from the carboxyl terminal of the canine SRbeta subunit

Isotype: IgG

Application Details

Application Notes: Western blot: 1 to 5 μg/mL. Immunohistochemistry. Positive control: Canine microsomal protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for SRPRB

Target: SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))

Alternative Name: SR-beta

Background: The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.Synonyms: APMCF1, PSEC0230, SR beta, SRPRB, Signal recognition particle receptor subunit beta

Gene ID: 58477

NCBI Accession: NP_067026

UniProt: Q9Y5M8

Pathways: ER-Nucleus Signaling