PARK7/DJ1 antibody

Catalog No. ABIN265384

This Rabbit Polyclonal antibody specifically detects PARK7/DJ1 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for PARK7/DJ1 antibody (ABIN265384)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARK7/DJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PARK7/DJ1 Antibody

Specificity: This antibody detects endogenous levels of DJ-1/PARK7 protein (region surrounding Pro54).

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Alternative Name: DJ-1 / PARK7

Background: The DJ-1 gene encodes a highly-conserved protein which is implicated in a number of cell processes. DJ-1 was first identified as a novel oncogene that transformed mouse NIH/3T3 cells in cooperation with activated Ras. Additionally, DJ-1 was cloned in rat as SP22 or CAP-1 and found to be an infertilityrelated sperm protein, whose expression is reduced in sperm treated with toxicants. DJ-1 also positively regulates the androgen receptor (AR) by forming a complex with PIASxα, a negative regulator of AR. The gene encoding human DJ-1 maps to chromosome 1p36.33-p36.12, a region identified as a hot spot of chromosome abnormalities in several tumor cells. Subsequently, mutations in the DJ-1 gene have been implicated in Parkinson's disease, and loss of DJ-1 function leads to neurodegeneration. DJ-1 is an ubiquitously expressed protein that is induced in response to growth stimuli and translocates from the cytoplasm to the nucleus during the S phase of the cell cycle.Synonyms: Oncogene DJ1, Parkinson disease protein 7

Molecular Weight: approx. 22 kDa

Gene ID: 11315

NCBI Accession: NP_001116849

UniProt: Q99497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport