Factor VIII antibody

Catalog No. ABIN265412

The Rabbit Polyclonal anti-Factor VIII antibody is suitable to detect Factor VIII in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for Factor VIII antibody (ABIN265412)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VIII antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Factor VIII Antibody

Specificity: This antibody detects endogenous levels of Coagulation factor VIII (F8) protein (region surronding Ser2194).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Background: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation Factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IX A, Factor X A, Factor XII A, or thrombin mediated proteolytic cleavage of Factor VII at Arg152-Ile153 generates Factor VII A, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing 2 EGF-like domains. Mutations at the F7 locus that lead to Factor VII deficiencies are generally asymptomatic or phenotypically uncharacterized, with hemorrhagic diathesis occurring at extremely low levels.Synonyms: AHF, Antihemophilic factor, F8C, Procoagulant component

Molecular Weight: approx. 267 kDa

Gene ID: 2157

NCBI Accession: NP_000123

UniProt: P00451