Recombinant ART2.2/Art2b antibody

Catalog No. ABIN2656684

The Mouse Monoclonal anti-ART2.2/Art2b antibody has been validated for ICFC. It is suitable to detect ART2.2/Art2b in samples from Mouse.

Quick Overview for Recombinant ART2.2/Art2b antibody (ABIN2656684)

Target: ART2.2/Art2b (Art2b) (ADP-Ribosyltransferase 2b (Art2b))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ART2.2/Art2b antibody is un-conjugated

Application: Intracellular Flow Cytometry (ICFC)

Clone: S-16a

Product Details anti-ART2.2/Art2b Antibody

Brand: Treg-Protector™

Characteristics: ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.

Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level: Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.

Immunogen: Expression vector encoding full length mouse ARTC2.

Isotype: Ig Fraction

Application Details

Application Notes: Treg-Protector™ (S+16a) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells.

Comment:

We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 1 mg/mL S-16a in PBS Treg-Protector ™ is sterilized by filtration through a 0.22 μm membrane and contains no preservatives.

Preservative: Without preservative

Handling Advice: Do not freeze. Keep sterile.

Storage: 4 °C

Storage Comment: Store between 2°C and 8°C. Do not freeze. Keep sterile.

Target Details for ART2.2/Art2b

Target: ART2.2/Art2b (Art2b) (ADP-Ribosyltransferase 2b (Art2b))

Alternative Name: ARTC2 Nanobody

Gene ID: 11872