TRA-2-49 antibody (Alexa Fluor 488)

Catalog No. ABIN2657107

The Mouse Monoclonal anti- antibody has been validated for FACS, Act, CMCD, Dep, IP and IHC. It is suitable to detect in samples from Human.

Quick Overview for TRA-2-49 antibody (Alexa Fluor 488) (ABIN2657107)

Target: TRA-2-49

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS), Activation (Act), Complement Mediated Cell Depletion (CMCD), Depletion (Dep), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: TRA-2-49-6E

Product Details

Purification: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details

Target: TRA-2-49

Background: TRA-2-49, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone, and kidney cells. It has been reported that the expression level of TRA-2-49 is decreased upon differentiation, therefore measurement of TRA-2-49 expression can be used to monitor the differentiation status of pluripotent stem cells.