CD86 antibody (Alexa Fluor 488)

Catalog No. ABIN2657484

This Mouse Monoclonal antibody specifically detects CD86 in FACS and ICFC. It exhibits reactivity toward Human.

Quick Overview for CD86 antibody (Alexa Fluor 488) (ABIN2657484)

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to Alexa Fluor 488

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: IT2-2

Product Details anti-CD86 Antibody (Alexa Fluor 488)

Cross-Reactivity: Baboon, Capuchin Monkey, Marmoset, Saguinus oedipus, Chimpanzee, Cynomolgus, Rhesus Monkey

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation