Panendothelial Cell Antigen antibody (Alexa Fluor 488)

Catalog No. ABIN2657569

The Rat Monoclonal anti-Panendothelial Cell Antigen antibody is suitable to detect Panendothelial Cell Antigen in samples from Mouse. It has been validated for FACS, IF and WB.

Quick Overview for Panendothelial Cell Antigen antibody (Alexa Fluor 488) (ABIN2657569)

Target: Panendothelial Cell Antigen

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: MECA-32

Product Details

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details

Target: Panendothelial Cell Antigen

Background: MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.