CD28 antibody (Alexa Fluor 700)

Catalog No. ABIN2658047

This anti-CD28 antibody is a Mouse Monoclonal antibody detecting CD28 in FACS, WB and IP. Suitable for Human.

Quick Overview for CD28 antibody (Alexa Fluor 700) (ABIN2658047)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to Alexa Fluor 700

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: CD28-2

Product Details anti-CD28 Antibody (Alexa Fluor 700)

Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Capuchin Monkey, Sooty mangabey (Cercocebus atys), Squirrel Monkey (Saimiri spec.)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response