CD43 antibody (APC)

Catalog No. ABIN2658232

The Rat Monoclonal anti-CD43 antibody has been validated for FACS. It is suitable to detect CD43 in samples from Mouse.

Quick Overview for CD43 antibody (APC) (ABIN2658232)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD43 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: S11

Product Details anti-CD43 Antibody (APC)

Purification: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: CD43, also known as Leukosialin and Ly48, is a 125 kD sialoprotein (glycosylated protein) expressed from 1.2 kBase mRNA in bone marrow-derived cells. This occurs early in development. Cells expressing CD43 include γ/δ T cells, macrophages, mature B cells, and dendritic cells. CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia. The intracellular distribution of CD43 is determined by binding to moesin, an intracellular membrane protein, which is in-turn bound in some manner to the actin cytoskeleton. Defects with CD43 function and expression retard cellular locomotion, resulting in a wide range of immune disorders. Wiscott-Alderich syndrome, and the varying degrees of its severity, is related to the dysregulation of CD43 expression.

Pathways: Regulation of Leukocyte Mediated Immunity