L-Selectin antibody (APC)

Catalog No. ABIN2658700

The Mouse Monoclonal anti-L-Selectin antibody has been validated for FACS and ICFC. It is suitable to detect L-Selectin in samples from Chemical.

Quick Overview for L-Selectin antibody (APC) (ABIN2658700)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is conjugated to APC

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: DREG-56

Product Details anti-L-Selectin Antibody (APC)

Cross-Reactivity: Chimpanzee, Cow (Bovine)

Purification: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L

Target Type: Chemical

Background: CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naive lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.