CD8 antibody (Biotin)

Catalog No. ABIN2660849

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in ICFC. Suitable for Human.

Quick Overview for CD8 antibody (Biotin) (ABIN2660849)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to Biotin

Application: Intracellular Flow Cytometry (ICFC)

Clone: SK1

Product Details anti-CD8 Antibody (Biotin)

Cross-Reactivity: Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops), Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus Monkey, Sooty mangabey (Cercocebus atys), Green Monkey (Chlorocebus sabaeus)

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.