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H-2Kk antibody (Biotin)

The Mouse Monoclonal anti-H-2Kk antibody has been validated for ICFC, WB, IF and IP. It is suitable to detect H-2Kk in samples from Mouse.
Catalog No. ABIN2661178

Quick Overview for H-2Kk antibody (Biotin) (ABIN2661178)

Target

H-2Kk (MHC, Class I H-2Kk)

Reactivity

Mouse

Host

  • 15
  • 4
Mouse

Clonality

  • 19
Monoclonal

Conjugate

  • 7
  • 5
  • 4
  • 3
This H-2Kk antibody is conjugated to Biotin

Application

  • 18
  • 8
  • 6
  • 4
  • 2
Intracellular Flow Cytometry (ICFC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone

36-7-5
  • Cross-Reactivity (Details)

    Mouse k haplotype, Weakly cross-reacts with SJL/Hsd mice splenocytes, but does not react with alloantigens of other haplotypes (e.g., b, d, q).

    Characteristics

    The 36-7-5 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.

    Purification

    The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

    Isotype

    IgG2a kappa
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.

    Storage

    4 °C

    Storage Comment

    The antibody solution should be stored undiluted between 2°C and 8°C.
  • Target

    H-2Kk (MHC, Class I H-2Kk)

    Alternative Name

    H-2Kk
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