CD301b/MGL2 antibody

Catalog No. ABIN2664539

This anti-CD301b/MGL2 antibody is a Rat Monoclonal antibody detecting CD301b/MGL2 in ELISA (Detection) and ELISPOT (Detection). Suitable for Mouse.

Quick Overview for CD301b/MGL2 antibody (ABIN2664539)

Target: CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD301b/MGL2 antibody is un-conjugated

Application: ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))

Clone: URA-1

Product Details anti-CD301b/MGL2 Antibody

Purification: The antibody was purified by affinity chromatography.

Isotype: IgG2a lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD301b/MGL2

Target: CD301b/MGL2 (MGL2) (Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2))

Alternative Name: CD301b

Background: Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92 % for the intact sequence and 80 % for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.