CD16 antibody

Catalog No. ABIN2665954

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Brand: Ultra-LEAF™

Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Marmoset, Capuchin Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys), Squirrel Monkey (Saimiri spec.), Saguinus oedipus

Purification: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level:

Endotoxin level is <0.01 EU/μg of the protein (<0.001 ng/μg of the protein) as determined by the LAL test.

Clone: 3G8

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Preservative: Azide free

Handling Advice: This Ultra-LEAF™ solution contains no preservative, handle under aseptic conditions

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Background: CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95 % homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process