IRF3 antibody

Catalog No. ABIN2666278

The Mouse Monoclonal anti-IRF3 antibody has been validated for BCA. It is suitable to detect IRF3 in samples from Human and Mouse.

Quick Overview for IRF3 antibody (ABIN2666278)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Biochemical Assay (BCA)

Clone: 12A4A35

Product Details anti-IRF3 Antibody

Purification: Affinity purified

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store undiluted between 2°C and 8°C.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3

Background: IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades