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Histone 3 antibody (H3K4me2)

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN and CUT&Tag. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.
Catalog No. ABIN2668392

Quick Overview for Histone 3 antibody (H3K4me2) (ABIN2668392)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse

Host

  • 1157
  • 250
  • 8
  • 5
Mouse

Clonality

  • 1075
  • 344
  • 1
Monoclonal

Conjugate

  • 814
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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)

Clone

MABI 0303
  • Binding Specificity

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    H3K4me2

    Purification

    Protein G Chromatography

    Immunogen

    This Histone H3 dimethylLys4 antibody was raised against a synthetic peptide containing dimethylLys4 of human histone H3.

    Isotype

    IgG1
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.46 μg/μL

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage

    -20 °C

    Storage Comment

    Antibodies in solution can be stored at -20 °C for 2 years.

    Expiry Date

    6 months
  • Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

    Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Molecular Weight

    17 kDa

    Gene ID

    3020
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