Histone 3 antibody (H3K27ac)

Catalog No. ABIN2668396

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K27ac

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Purification: Protein G Chromatography

Immunogen: This Histone H3 acetylLys27 antibody was raised against a synthetic peptide including acetyllysine 27 of human histone H3.

Clone: MABI 0309

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.43 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668396)

Dheekollu, Wiedmer, Sentana-Lledo, Cassel, Messick, Lieberman: "HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus." in: Journal of virology, Vol. 90, Issue 11, pp. 5353-67, (2016) (PubMed).

Kotomura, Harada, Ishihara: "The Proportion of Chromatin Graded between Closed and Open States Determines the Level of Transcripts Derived from Distinct Promoters in the CYP19 Gene." in: PLoS ONE, Vol. 10, Issue 5, pp. e0128282, (2015) (PubMed).

Gong, Qian, Ertl, Astle, Wang, Harrison, Xu: "Histone modifications change with age, dietary restriction and rapamycin treatment in mouse brain." in: Oncotarget, Vol. 6, Issue 18, pp. 15882-90, (2015) (PubMed).

Chang, Huang, Han, Jiang, Fang, Song, Padilla, Wang, Qu, Stamatoyannopoulos, Li, Papayannopoulou et al.: "Transcriptional environment and chromatin architecture interplay dictates globin expression patterns of heterospecific hybrids derived from undifferentiated human embryonic stem cells or from their ..." in: Experimental hematology, Vol. 41, Issue 11, pp. 967-979.e6, (2013) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020