O-GlcNAc antibody

Catalog No. ABIN2668989

This Mouse Monoclonal antibody specifically detects in WB and IP. It exhibits reactivity toward Chemical and has been mentioned in 2+ publications.

Quick Overview for O-GlcNAc antibody (ABIN2668989)

Target: O-GlcNAc

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: CTD110-6

Product Details

Purification: IgM purification

Immunogen: This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and threonine.

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References

Teo, Ingale, Wolfert, Elsayed, Nöt, Chatham, Wells, Boons: "Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." in: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).

Comer, Vosseller, Wells, Accavitti, Hart: "Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine." in: Analytical biochemistry, Vol. 293, Issue 2, pp. 169-77, (2001) (PubMed).

Target Details

Target: O-GlcNAc

Target Type: Viral Protein

Background: O-GlcNAc (b-O-linked N-acetylglucosamine) is a ubiquitous and dynamic post-translational modification present on numerous cytoplasmic and nuclear proteins and is thought to play a regulatory role in numerous cellular processes including homeostatic mechanisms and gene expression. OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residues whereas OGA / O-GlcNAcase (O- linked N-acetylglucosamine (GlcNAc)-ase) is a glycosidase that removes O-GlcNAc. O-GlcNAc modifications have been described to directly regulate the activities of a variety of transcription factors including Sp1, estrogen receptors, STAT5, NF-κB, p53, YY1, Elf-1, c-Myc, Rb, PDX-1, CREB, fork-head, and others. In addition, RNA polymerase II and the basal transcription factors are also extensively modified by O-GlcNAc. O-GlcNAc modification has been reported to suppress or enhance transcription, depending on the promoter and other associated proteins.