This Rabbit Polyclonal antibody specifically detects POR in WB. It exhibits reactivity toward Wheat, Pisum sativum, Oryza sativa, Nicotiana tabacum, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum) and Arabidopsis thaliana and has been mentioned in 3+ publications.
POR
Reactivity: Human, Rat, Mouse
WB, IHC, FACS, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Recommended Dilution: 1 : 2 000 with standard ECL (WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL, of sterile water.
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Yuan, Zhang, Zhang, Chen, Yuan, Guo, Lin: "Assembly of NADPH: protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings." in: Journal of plant physiology, Vol. 169, Issue 13, pp. 1311-6, (2012) (PubMed).
Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).
Wei, Zhou, Hu, Wei, Yang, Huang: "Heterotrimeric G-protein is involved in phytochrome A-mediated cell death of Arabidopsis hypocotyls." in: Cell research, (2008) (PubMed).
Target
POR
(P450 (Cytochrome) Oxidoreductase (POR))
Alternative Name
POR
Background
Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.