Alkaline Phosphatase antibody

Catalog No. ABIN2688842

Product Details anti-Alkaline Phosphatase Antibody

Target: Alkaline Phosphatase (ALP)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody (solid line) or a Purified mouse IgG1, κ isotype control (

BD Pharmingen™ Purified Mouse anti-Human Alkaline Phosphatase - Purified - Clone B4-78 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Bone Alkaline Phosphatase

Clone: B4

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Alkaline Phosphatase antibody (ABIN2688842)

OConnor, Kardel, Iosfina, Youssef, Lu, Li, Vercauteren, Nagy, Eaves: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).

Adewumi, Aflatoonian, Ahrlund-Richter, Amit, Andrews, Beighton, Bello, Benvenisty, Berry, Bevan, Blum, Brooking, Chen, Choo, Churchill, Corbel, Damjanov, Draper, Dvorak, Emanuelsson, Fleck, Ford et al.: "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. ..." in: Nature biotechnology, Vol. 25, Issue 7, pp. 803-16, (2007) (PubMed).

Karp, Ferreira, Khademhosseini, Kwon, Yeh, Langer: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, (2006) (PubMed).

Eghbali-Fatourechi, Lamsam, Fraser, Nagel, Riggs, Khosla: "Circulating osteoblast-lineage cells in humans." in: The New England journal of medicine, Vol. 352, Issue 19, pp. 1959-66, (2005) (PubMed).

Dorheim, Sullivan, Dandapani, Wu, Hudson, Segarini, Rosen, Aulthouse, Gimble: "Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells." in: Journal of cellular physiology, Vol. 154, Issue 2, pp. 317-28, (1993) (PubMed).

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase (ALP Products)

Background: Synonyms: Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL Entrez Gene ID: 249