Alkaline Phosphatase antibody

Catalog No. ABIN2688842

This Mouse Monoclonal antibody specifically detects Alkaline Phosphatase in WB, IF, FACS, IP and BI. It exhibits reactivity toward Human and Mouse and has been mentioned in 5+ publications.

Quick Overview for Alkaline Phosphatase antibody (ABIN2688842)

Target: Alkaline Phosphatase (ALP)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), BioImaging (BI)

Clone: B4

Product Details anti-Alkaline Phosphatase Antibody

Brand: BD Pharmingen™

Characteristics: The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody (solid line) or a Purified mouse IgG1, κ isotype control (

BD Pharmingen™ Purified Mouse anti-Human Alkaline Phosphatase - Purified - Clone B4-78 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Bone Alkaline Phosphatase

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Alkaline Phosphatase antibody (ABIN2688842)

OConnor, Kardel, Iosfina, Youssef, Lu, Li, Vercauteren, Nagy, Eaves: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).

Adewumi, Aflatoonian, Ahrlund-Richter, Amit, Andrews, Beighton, Bello, Benvenisty, Berry, Bevan, Blum, Brooking, Chen, Choo, Churchill, Corbel, Damjanov, Draper, Dvorak, Emanuelsson, Fleck, Ford et al.: "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. ..." in: Nature biotechnology, Vol. 25, Issue 7, pp. 803-16, (2007) (PubMed).

Karp, Ferreira, Khademhosseini, Kwon, Yeh, Langer: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, (2006) (PubMed).

Eghbali-Fatourechi, Lamsam, Fraser, Nagel, Riggs, Khosla: "Circulating osteoblast-lineage cells in humans." in: The New England journal of medicine, Vol. 352, Issue 19, pp. 1959-66, (2005) (PubMed).

Dorheim, Sullivan, Dandapani, Wu, Hudson, Segarini, Rosen, Aulthouse, Gimble: "Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells." in: Journal of cellular physiology, Vol. 154, Issue 2, pp. 317-28, (1993) (PubMed).

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background: Synonyms: Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL Entrez Gene ID: 249