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Basophils antibody

This Mouse Monoclonal antibody specifically detects Basophils in FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.
Catalog No. ABIN2688848

Quick Overview for Basophils antibody (ABIN2688848)

Target

Basophils

Reactivity

Human

Host

  • 1
Mouse

Clonality

  • 1
Monoclonal

Conjugate

  • 1
This Basophils antibody is un-conjugated

Application

  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

Bsp
  • Brand

    BD Pharmingen™

    Characteristics

    Bsp-1 reacts with a 45 kDa surface antigen on the human erythroleukemic (HEL) cell line, the KU812 basophilic cell line, and peripheral blood basophils, but not on neutrophils or eosinophils. Bsp-1+ basophils also express CD11 and CD13, but lack lymphoid and other granulocytic/monocytic cell surface antigens. However, they express CD45 and HLA-ABC antigens. In peripheral blood, Bsp-1 reacts with a subset of the CD123+/HLA-DR- population. Bsp-1 is a useful tool in the study of basophil function and differentiation. Profile of anti-Basophil (Bsp-1) on HEL cells analyzed by flow cytometry. Second step staining with Cat. No. 555988.

    BD Pharmingen™ Purified Mouse Anti-Human Basophils - Purified - Clone Bsp-1 - Isotype Mouse IgM, κ - Reactivity Hu - 0.1 mg

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Isotype

    IgM kappa
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Aqueous buffered solution containing ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store undiluted at 4°C.
  • Bodger, Morris, Kennedy, Bowen, Hilton, Fitzgerald: "Basophils (Bsp-1+) derive from the leukemic clone in human myeloid leukemias involving the chromosome breakpoint 9q34." in: Blood, Vol. 73, Issue 3, pp. 777-81, (1989) (PubMed).

    Bodger, Mounsey, Nelson, Fitzgerald: "A monoclonal antibody reacting with human basophils." in: Blood, Vol. 69, Issue 5, pp. 1414-8, (1987) (PubMed).

  • Target

    Basophils
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