C3a antibody

Catalog No. ABIN2688860

This Rat Monoclonal antibody specifically detects C3a in ELISA. It exhibits reactivity toward Mouse. It has been mentioned in 2+ publications

Quick Overview for C3a antibody (ABIN2688860)

Target: C3a (Complement Fragment 3a (C3a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This C3a antibody is un-conjugated

Application: ELISA

Clone: I87

Product Details anti-C3a Antibody

Characteristics: The purified I87-1162 antibody reacts with the mouse C3a protein. The I87-1162 antibody is specific for a neoepitope exposed in mouse C3a/C3adesArg and does not cross-react with mouse C3. This capacity makes antibody I87-1162 a preferential capture antibody for direct detection of mouse C3a from plasma or serum samples in an ELISA sandwich assay. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation. Representative Mouse C3a standard curve. The curve was generated by a sandwich ELISA using the purified I87-1162 antibody (Cat. No. 558250) as capture antibody, doubling dilutions of purified mouse C3a protein (Cat. No. 558618) as standard, and biotinylated I87-419 antibody (Cat. No. 558251) as detection antibody. The standard curve is displayed as the concentration of purified mouse C3a in ng/mL versus the microwell absorbance.

BD Pharmingen™ Purified Rat Anti-Mouse C3a - Purified - Clone I87-1162 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: ELISA) Hugli T.E. Structure and function of the anaphylatoxins.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-C3a antibody (ABIN2688860)

Hugli: "Structure and function of C3a anaphylatoxin." in: Current topics in microbiology and immunology, Vol. 153, pp. 181-208, (1990) (PubMed).

Hugli: "Structure and function of the anaphylatoxins." in: Springer seminars in immunopathology, Vol. 7, Issue 2-3, pp. 193-219, (1984) (PubMed).

Target Details for C3a

Target: C3a (Complement Fragment 3a (C3a))

Alternative Name: C3a

Pathways: Complement System