IFNGR1 antibody

Catalog No. ABIN2688896

This Mouse Monoclonal antibody specifically detects IFNGR1 in FACS and IF. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for IFNGR1 antibody (ABIN2688896)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNGR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GIR

Product Details anti-IFNGR1 Antibody

Brand: BD Pharmingen™

Characteristics: The GIR-94 antibody recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses. The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα purified from human placenta. The GIR-94 is a non-neutralizing antibody. Human PBMC (Left panel) and red blood cells (Right panel) were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with GIR-94 (1 μg, Cat. No. 558935) followed by biotinylated goat anti- mouse IgG (0.25 μg, Cat. No. 553999) and streptavidin -PE (0.015 μg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the GIR-94 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in left panel are gated on the lymphocyte population defined by its light scattering characteristics.

BD Pharmingen™ Purified Mouse Anti-Human CD119 - Purified - Clone GIR-94 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IFN-gammaRalpha

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IFNGR1 antibody (ABIN2688896)

Bach, Aguet, Schreiber: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." in: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).

Greenlund, Schreiber, Goeddel, Pennica: "Interferon-gamma induces receptor dimerization in solution and on cells." in: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18103-10, (1993) (PubMed).

Sheehan, Calderon, Schreiber: "Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4231-7, (1988) (PubMed).

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119

Background: Synonyms: IFN-γ Receptor α chain

Pathways: Interferon-gamma Pathway