IFNGR1 antibody (Biotin)

Catalog No. ABIN2688897

There is 1 publication for this product available. The Armenian Hamster Monoclonal anti-IFNGR1 antibody is suitable to detect IFNGR1 in samples from Mouse. It has been validated for FACS.

Quick Overview for IFNGR1 antibody (Biotin) (ABIN2688897)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IFNGR1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 2E2

Product Details anti-IFNGR1 Antibody (Biotin)

Characteristics: The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the mouse IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γRα and IFN-γRβ chains required for the transduction of biologic responses. IFN-γRα is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-γ to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-γRα chain protein. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of cell surface IFN-γRα by BALB/c splenic lymphocytes. RBC-lysed BALB/c spleen cells were preincubated (~15 minutes, 4 °C) with purified 2.4G2 antibody [rat anti-mouse CD16 (FcγIII)/CD32 (FcγII), Cat. No. 553142, 1 μg antibody/10e6 cells]. The cells were stained (30 minutes, 4 °C) with biotinylated 2E2 antibody (1 μg mAb/10e6 cells, Cat. No. 550482) followed by R-PE-conjugated streptavidin (Cat. No. 554061, 0.015 μg PE-SA/10e6 cells). After washing, the cells were analyzed with a FACScan™ Flow Cytometer. The immunofluorescent staining patterns for cells stained with either biotinylated 2E2 antibody (filled histogram) or Streptavidin-PE (background staining, empty histogram) are shown. The histograms were generated from reanalyzed flow cytometric data files that were gated for events with the light-scattering characteristics of lymphocytes.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD119 - Biotin - Clone 2E2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IFNGR1 antibody (Biotin) (ABIN2688897)

Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119

Background: Synonyms: IFN-γ Receptor α chain

Pathways: Interferon-gamma Pathway