IL4 Receptor antibody (Biotin)

Catalog No. ABIN2688938

This Rat Monoclonal antibody specifically detects IL4 Receptor in ELISA, FACS, IP and Neut. It exhibits reactivity toward Mouse and has been mentioned in 4+ publications.

Quick Overview for IL4 Receptor antibody (Biotin) (ABIN2688938)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut)

Clone: MIL4R

Product Details anti-IL4 Receptor Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block™ (Cat. No. 553142) to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 (0.125 μg/10^6 cells) followed by streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) and FITC anti-mouse B220 (0.06 μg, Cat. No. 553088) and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells (with the light-scattering characteristics of viable lymphocytes) are shown in the two-color dot plot (right panel). Staining with the biotinylated mIL4R-M1 antibody (right panel) is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody (0.125 μg, Cat. No. 553928) that is shown in the left panel.

BD Pharmingen™ Biotin Rat Anti-Mouse CD124 - Biotin - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CTLL-19.4 T Cell Line

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL4 Receptor antibody (Biotin) (ABIN2688938)

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, (1994) (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: CD124

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process