CTLA4 antibody (Biotin)

Catalog No. ABIN2688990

The Mouse Monoclonal anti-CTLA4 antibody has been validated for FACS. It is suitable to detect CTLA4 in samples from Human. There are 4+ publications available.

Quick Overview for CTLA4 antibody (Biotin) (ABIN2688990)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: BNI3

Product Details anti-CTLA4 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: Reacts with the "cytolytic T lymphocyte-associated antigen", CTLA-4. CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports that indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a possible role of CTLA-4 in the regulation of B-cell response. Immobilized BNI3.1 enhances T-cell proliferation induced by CD3 and CD28. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Concanavalin A-stimulated peripheral blood mononuclear cells analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD152 - Biotin - Clone BNI3 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CTLA4 antibody (Biotin) (ABIN2688990)

Morton, Fu, Stewart, Giacoletto, White, Leysath, Evans, Shieh, Karr: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, (1996) (PubMed).

Kuiper, Brouwer, Linsley, van Lier: "Activated T cells can induce high levels of CTLA-4 expression on B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 4, pp. 1776-83, (1995) (PubMed).

Lindsten, Lee, Harris, Petryniak, Craighead, Reynolds, Lombard, Freeman, Nadler, Gray: "Characterization of CTLA-4 structure and expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, (1993) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152

Background: Synonyms: CTLA-4

Pathways: Cancer Immune Checkpoints