CD40 Ligand antibody

Catalog No. ABIN2688992

This Mouse Monoclonal antibody specifically detects CD40 Ligand in FACS. It exhibits reactivity toward Human, Rhesus Monkey, Baboon and Cynomolgus and has been mentioned in 4+ publications.

Quick Overview for CD40 Ligand antibody (ABIN2688992)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Rhesus Monkey, Baboon, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: TRAP1

Product Details anti-CD40 Ligand Antibody

Characteristics: Reacts with CD40 ligand (CD40L), a 39 kDa type II membrane glycoprotein expressed on activated T cells. CD40-CD40L interaction plays a very important role in T cell-dependent B-cell proliferation, differentiation, and memory cell formation. Anti-CD40L mAb partially blocks T cell-B cell interaction affecting subsequent proliferation, differentiation, and memory cell formation. Anti-CD40L mAb partially blocks T cell-B cell interaction affecting subsequent proliferation, IL-2R expression and differentiation of B cells. In addition, blocking of CD40-CD40L interaction has been demonstrated with soluble CD40, resulting in the inhibition of immunoglobulin isotype switching. It has been reported that patients with X-linked hyper-IgM syndrome have defective expression of functional CD40L due to a defective gene that encodes CD40L. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of TPA+Ca++ Ionophore-stimulated PBMCs analyzed on a FACScan

BD Pharmingen™ Purified Mouse Anti-Human CD154 - Purified - Clone TRAP1 (also known as TRAP-1) - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD40 Ligand antibody (ABIN2688992)

Fuleihan, Ramesh, Horner, Ahern, Belshaw, Alberg, Stamenkovic, Harmon, Geha: "Cyclosporin A inhibits CD40 ligand expression in T lymphocytes." in: The Journal of clinical investigation, Vol. 93, Issue 3, pp. 1315-20, (1994) (PubMed).

Gray, Dullforce, Jainandunsing: "Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 141-55, (1994) (PubMed).

Nishioka, Lipsky: "The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1027-36, (1994) (PubMed).

van Kooten, Gaillard, Galizzi, Hermann, Fossiez, Banchereau, Blanchard: "B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40." in: European journal of immunology, Vol. 24, Issue 4, pp. 787-92, (1994) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154

Background: Synonyms: CD40L

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints