SIRPA antibody

Catalog No. ABIN2689017

The Rat Monoclonal anti-SIRPA antibody has been validated for WB, FACS, IP, IF and BR. It is suitable to detect SIRPA in samples from Mouse. There are 7+ publications available.

Quick Overview for SIRPA antibody (ABIN2689017)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR)

Clone: P84

Product Details anti-SIRPA Antibody

Brand: BD Pharmingen™

Characteristics: The P84 antibody reacts with CD172a, also known as SIgnal-Regulatory Protein α (SIRPα), Src Homology 2 domain-containing protein tyrosine Phosphatase (SHP) Substrate 1 (SHPS-1), or Brain Immunoglobulin-like molecule with Tyrosine-based activation motifs (BIT). CD172a is an adhesion molecule of the Ig superfamily which is expressed on neurons in the central nervous system and the retina, on macrophages, and on bone-marrow myeloid cells. Its ligand, CD47, or Integrin-Associated Protein (IAP), is expressed by a wide variety of cells. CD172a and CD47 are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. Two-color analysis of CD172a expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG1 isotype control mAb (left panel) or purified mAb P84 (right panel), followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892, both panels), then PE-conjugated anti-mouse CD11b (Mac-1) mAb M1/70 (Cat. No. 557397/553311). Total viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibura .

BD Pharmingen™ Purified Rat Anti-Mouse CD172a - Purified - Clone P84 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Membrane fractions from mouse brain

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SIRPA antibody (ABIN2689017)

Mi, Jiang, Weng, Lindberg, Narayanan, Lagenaur: "Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina." in: The Journal of comparative neurology, Vol. 416, Issue 3, pp. 335-44, (2000) (PubMed).

Gresham, Dale, Potter, Chang, Vines, Lowell, Lagenaur, Willman: "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." in: The Journal of experimental medicine, Vol. 191, Issue 3, pp. 515-28, (2000) (PubMed).

Oldenborg, Zheleznyak, Fang, Lagenaur, Gresham, Lindberg: "Role of CD47 as a marker of self on red blood cells." in: Science (New York, N.Y.), Vol. 288, Issue 5473, pp. 2051-4, (2000) (PubMed).

Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, (1999) (PubMed).

Veillette, Thibaudeau, Latour: "High expression of inhibitory receptor SHPS-1 and its association with protein-tyrosine phosphatase SHP-1 in macrophages." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22719-28, (1998) (PubMed).

Comu, Weng, Olinsky, Ishwad, Mi, Hempel, Watkins, Lagenaur, Narayanan: "The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 22, pp. 8702-10, (1997) (PubMed).

Chuang, Lagenaur: "Central nervous system antigen P84 can serve as a substrate for neurite outgrowth." in: Developmental biology, Vol. 137, Issue 2, pp. 219-32, (1990) (PubMed).

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background: Synonyms: SIRPα, SHPS-1, BIT