CD1d antibody (Biotin)

Catalog No. ABIN2689069

This anti-CD1d antibody is a Rat Monoclonal antibody detecting CD1d in FACS, IF and IHC (fro). Suitable for Mouse. This Primary Antibody has been cited in 9+ publications.

Quick Overview for CD1d antibody (Biotin) (ABIN2689069)

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD1d antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1B1

Product Details anti-CD1d Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The 1B1 antibody reacts with CD1d, a 48- kDa glycoprotein with structural homology to the major histocompatibility complex (MHC) class I molecules. The structure, expression, and functions of CD1 antigens are complex and have been reviewed. mAb 1B1 detects CD1d at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus. It appears to recognize CD1d only in association with β2m. CD1d has been reported to be expressed by gastrointestinal tract epithelium and in the cytoplasm of hepatocytes as detected viaimmunohistochemical staining of frozen sections with mAb 3C11 (Cat. No. 559871, for the purified antibody), suggesting a possible role for CD1d in mucosal immunity. However, CD1d expression was not detectable, via flow cytometry, on intestinal epithelial cells in studies using the anti-CD1d mAbs 3C11, 1B1, and 9C7. The 1B1 antibody competes with mAb 3C11 in binding to mouse splenocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD1d - Biotin - Clone 1B1 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma and mouse L929 cells

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD1d antibody (Biotin) (ABIN2689069)

Prigozy, Naidenko, Qasba, Elewaut, Brossay, Khurana, Natori, Koezuka, Kulkarni, Kronenberg: "Glycolipid antigen processing for presentation by CD1d molecules." in: Science (New York, N.Y.), Vol. 291, Issue 5504, pp. 664-7, (2001) (PubMed).

Porcelli, Modlin: "The CD1 system: antigen-presenting molecules for T cell recognition of lipids and glycolipids." in: Annual review of immunology, Vol. 17, pp. 297-329, (1999) (PubMed).

Szalay, Ladel, Blum, Brossay, Kronenberg, Kaufmann: "Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6955-8, (1999) (PubMed).

Brossay, Tangri, Bix, Cardell, Locksley, Kronenberg: "Mouse CD1-autoreactive T cells have diverse patterns of reactivity to CD1+ targets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 8, pp. 3681-8, (1998) (PubMed).

Amano, Baumgarth, Dick, Brossay, Kronenberg, Herzenberg, Strober: "CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 4, pp. 1710-7, (1998) (PubMed).

Roark, Park, Jayawardena, Kavita, Shannon, Bendelac: "CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3121-7, (1998) (PubMed).

Brossay, Jullien, Cardell, Sydora, Burdin, Modlin, Kronenberg: "Mouse CD1 is mainly expressed on hemopoietic-derived cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 3, pp. 1216-24, (1997) (PubMed).

Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).

Bleicher, Balk, Hagen, Blumberg, Flotte, Terhorst: "Expression of murine CD1 on gastrointestinal epithelium." in: Science (New York, N.Y.), Vol. 250, Issue 4981, pp. 679-82, (1990) (PubMed).

Target Details for CD1d

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Alternative Name: CD1d

Background: Synonyms: CD1.1, Ly-38

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process