2B4 antibody

Catalog No. ABIN2689112

This Rat Monoclonal antibody specifically detects 2B4 in FACS and IP. It exhibits reactivity toward Mouse and has been mentioned in 7+ publications.

Quick Overview for 2B4 antibody (ABIN2689112)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: C9-1

Product Details anti-2B4 Antibody

Brand: BD Pharmingen™

Characteristics: CD244 (also known as 2B4 Antigen) is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).3,4,5 It is expressed on all natural killer (NK) cells, IL-2-activated NK cells (LAK), and a subset of T lymphocytes which mediate non-MHC-restricted cytotoxicity. The C9.1 antibody reacts with CD244.1, the 2B4 alloantigen which is expressed in most strains tested (A/J, AKR/N, BALB/c, CBA/N, C3H/He, DBA/1, DBA/2, NZB/N, SJL/J, 129/Svj), but not C57BL/6 or C58/J mice. The expression of the CD244.1 alloantigen on B lymphocytes that was originally described, has not been confirmed in later studies. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. Two-color analysis of CD244.1 expression on splenic NK cells. BALB/c splenocytes were stained with either purified rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553986, left panel) or purified mAb C9.1 (right panel), followed by FITC-conjugated anti-rat IgG2b mAb RG7/11.1 (Cat. No. 553900) then PE-conjugated anti-mouse CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD244.1 - Purified - Clone C9.1 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse MHC-unrestricted killer hybridoma 7D4

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-2B4 antibody (ABIN2689112)

Kumaresan, Huynh, Mathew: "Polymorphism in the 2B4 gene of inbred mouse strains." in: Immunogenetics, Vol. 51, Issue 8-9, pp. 758-61, (2000) (PubMed).

Kubota, Katoh, Muguruma, Koyama: "Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen." in: Immunology, Vol. 96, Issue 3, pp. 491-7, (1999) (PubMed).

Brown, Boles, van der Merwe, Kumar, Mathew, Barclay: "2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2083-90, (1999) (PubMed).

Latchman, McKay, Reiser: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, (1998) (PubMed).

Kubota: "A killer cell protective antigen expressed by MHC-unrestricted killer hybridomas." in: Cellular immunology, Vol. 181, Issue 1, pp. 50-8, (1997) (PubMed).

Davis, van der Merwe: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, (1997) (PubMed).

Mathew, Garni-Wagner, Land, Takashima, Stoneman, Bennett, Kumar: "Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5328-37, (1993) (PubMed).

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244.1

Background: Synonyms: 2B4 BALB Alloantigen