TRAIL antibody

Catalog No. ABIN2689128

The Rat Monoclonal anti-TRAIL antibody has been validated for WB and FACS. It is suitable to detect TRAIL in samples from Mouse. There are 4+ publications available.

Quick Overview for TRAIL antibody (ABIN2689128)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TRAIL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: N2B2

Product Details anti-TRAIL Antibody

Brand: BD Pharmingen™

Characteristics: The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40- kDa type-II transmembrane protein of the TNF family. This antibody recognizes mouse Trail-transfected 2PK-3 cells, but not the parental cell line. TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells. Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far. The pattern of expression of mouse TRAIL is controversial. TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines. Similar results were obtained by western blot and mRNA analyses. However, these results could not be reproduced with the N2B2 mAb. TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15. However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface. Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb. TRAIL has been renamed as CD253 recently.

BD Pharmingen™ Purified Rat Anti-Mouse CD253 - Purified - Clone N2B2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Trail-transfected 2PK-3 cells

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TRAIL antibody (ABIN2689128)

Degli-Esposti: "To die or not to die--the quest of the TRAIL receptors." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 535-42, (1999) (PubMed).

Kayagaki, Yamaguchi, Nakayama, Takeda, Akiba, Tsutsui, Okamura, Nakanishi, Okumura, Yagita: "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 1906-13, (1999) (PubMed).

Mariani, Krammer: "Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells." in: European journal of immunology, Vol. 28, Issue 5, pp. 1492-8, (1998) (PubMed).

Mariani, Krammer: "Differential regulation of TRAIL and CD95 ligand in transformed cells of the T and B lymphocyte lineage." in: European journal of immunology, Vol. 28, Issue 3, pp. 973-82, (1998) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: CD253

Background: Synonyms: TRAIL, APO-2L, Ly-81, TL2

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity