CD44 Standard antibody

Catalog No. ABIN2689266

There are 7+ publications for this product available. The Mouse Monoclonal anti-CD44 Standard antibody is suitable to detect CD44 Standard in samples from Rat. It has been validated for FACS, IHC (f), IP, IHC (fro) and IHC (zinc).

Quick Overview for CD44 Standard antibody (ABIN2689266)

Target: CD44 Standard (CD44s)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 Standard antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: OX

Product Details anti-CD44 Standard Antibody

Characteristics: The OX-49 antibody reacts with the glycoprotein CD44H (also known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes, and at greatly increased levels on T- and B-cell blasts. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However, recent reports indicate that OX-49 antibody cannot detect the CD44v isoform, possibly due to conformational changes in the epitope. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices.

BD Pharmingen™ Purified Mouse Anti-Rat CD44H - Purified - Clone OX-49 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat T blasts from mixed lymphocyte reactions

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD44 Standard antibody (ABIN2689266)

Noonan, Stevens, Tammi, Tammi, Hernandez, Midura: "Spatial distribution of CD44 and hyaluronan in the proximal tibia of the growing rat." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 14, Issue 4, pp. 573-81, (1996) (PubMed).

Stevens, Noonan, Bosch, Rapp, Martin, Kurriger, Maynard, Daniels, Solursh, Tammi, Tammi, Midura: "CD44 in growing normal and neoplastic rat cartilage." in: Annals of the New York Academy of Sciences, Vol. 785, pp. 333-6, (1996) (PubMed).

Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, (1995) (PubMed).

Westermann, Nagahori, Walter, Heerwagen, Miyasaka, Pabst et al.: "B and T lymphocyte subsets enter peripheral lymph nodes and Peyer's patches without preference in vivo: no correlation occurs between their localization in different types of high endothelial venules ..." in: European journal of immunology, Vol. 24, Issue 10, pp. 2312-6, (1994) (PubMed).

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

Arch, Wirth, Hofmann, Ponta, Matzku, Herrlich, Zöller: "Participation in normal immune responses of a metastasis-inducing splice variant of CD44." in: Science (New York, N.Y.), Vol. 257, Issue 5070, pp. 682-5, (1992) (PubMed).

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, (1988) (PubMed).

Target Details for CD44 Standard

Target: CD44 Standard (CD44s)

Alternative Name: CD44H

Background: Synonyms: Pgp-1, H-CAM, CD44s