ICAM1 antibody

Catalog No. ABIN2689330

This anti-ICAM1 antibody is a Armenian Hamster Monoclonal antibody detecting ICAM1 in FACS, IHC (fro) and BR. Suitable for Mouse. This Primary Antibody has been cited in 11+ publications.

Quick Overview for ICAM1 antibody (ABIN2689330)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: 3E2

Product Details anti-ICAM1 Antibody

Brand: BD Pharmingen™

Characteristics: The 3E2 antibody reacts with CD54 (ICAM-1), a 95- kDa member of the Ig superfamily found on lymphocytes, vascular endothelium, high endothelial venules, epithelial cells, macrophages, and dendritic cells. ICAM-1 is a ligand for LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated upon stimulation by inflammatory mediators such as cytokines and LPS. Studies with mouse Icam1-transfected antigen-presenting cells, with CD54-blocking antibodies, and in CD54-deficient mice indicate that CD54 participates in inflammatory reactions and antigen-specific immune responses. In addition, there is evidence that CD54 is a receptor involved in MHC-non-restricted responses to weakly immunogenic tumor cells. The 3E2 antibody blocks in vitro and in responses. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel: Naive BALB/c splenocytes were stained with purified 3E2 mAb (open histogram) followed by FITC-conjugated anti-hamster IgG cocktail (Cat. No. 554011, filled and open histograms). Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD (BD Via-Probe™, Cat. No. 555816/555815). The mean fluorescence intensity of the stained lymphocytes is about 7.5 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with purified 3E2 mAb (open histogram) followed by FITC-conjugated anti-hamster IgG (filled and open histograms). Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 15 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD54 - Purified - Clone 3E2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not reported

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ICAM1 antibody (ABIN2689330)

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Gonzalo, Martinez, Springer, Gutierrez-Ramos: "ICAM-1 is required for T cell proliferation but not for anergy or apoptosis induced by Staphylococcus aureus enterotoxin B in vivo." in: International immunology, Vol. 7, Issue 10, pp. 1691-8, (1996) (PubMed).

Kelly, Williams, Colvin, Meehan, Springer, Gutierrez-Ramos, Bonventre: "Intercellular adhesion molecule-1-deficient mice are protected against ischemic renal injury." in: The Journal of clinical investigation, Vol. 97, Issue 4, pp. 1056-63, (1996) (PubMed).

Nishio, Podack: "Rapid induction of tumor necrosis factor cytotoxicity in naive splenic T cells by simultaneous CD80 (B7.1) and CD54 (ICAM-1) co-stimulation." in: European journal of immunology, Vol. 26, Issue 9, pp. 2160-4, (1996) (PubMed).

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).

Soriano, Lipton, Wang, Xiao, Springer, Gutierrez-Ramos, Hickey: "Intercellular adhesion molecule-1-deficient mice are less susceptible to cerebral ischemia-reperfusion injury." in: Annals of neurology, Vol. 39, Issue 5, pp. 618-24, (1996) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Xu, Gonzalo, St Pierre, Williams, Kupper, Cotran, Springer, Gutierrez-Ramos: "Leukocytosis and resistance to septic shock in intercellular adhesion molecule 1-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 95-109, (1994) (PubMed).

Isobe, Yagita, Okumura, Ihara: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." in: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, (1992) (PubMed).

Siu, Hedrick, Brian: "Isolation of the murine intercellular adhesion molecule 1 (ICAM-1) gene. ICAM-1 enhances antigen-specific T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 11, pp. 3813-20, (1990) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: Synonyms: ICAM-1

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling