Integrin beta 3 antibody

Catalog No. ABIN2689351

This anti-Integrin beta 3 antibody is a Mouse Monoclonal antibody detecting Integrin beta 3 in FACS, IHC (fro), IP, IHC (f), BR and IHC (zinc). Suitable for Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Integrin beta 3 antibody (ABIN2689351)

Target: Integrin beta 3 (ITGB3)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: F11

Product Details anti-Integrin beta 3 Antibody

Brand: BD Pharmingen™

Characteristics: The F11 antibody reacts with the integrin β3 chain (CD61), which associates with the integrin αv chain (CD51), to form the vitronectin receptor found on endothelial cells, myeloid cells, and osteoclasts, and with the αIIb chain (CD41) on platelets and megakaryocytes. Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. F11 mAb has been reported to block the in vitro attachment of osteoclasts to several ligands. Weak reactivity with human osteoclasts, megakaryocytes, and platelets has also been observed. Other reported applications include immunoprecipitation, in vitro blocking, and immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections. IHC of formalin-fixed paraffin-embedded sections is not recommended.

BD Pharmingen™ Purified Mouse Anti-Rat CD61 - Purified - Clone F11 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat bone cell suspensions

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Integrin beta 3 antibody (ABIN2689351)

Helfrich, Nesbitt, Dorey, Horton: "Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a beta 3 integrin." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 7, Issue 3, pp. 335-43, (1992) (PubMed).

Helfrich, Nesbitt, Horton: "Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 7, Issue 3, pp. 345-51, (1992) (PubMed).

Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." in: Annual review of cell biology, Vol. 6, pp. 329-57, (1991) (PubMed).

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61

Background: Synonyms: Integrin β3 chain

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex