Transferrin Receptor antibody (Biotin)

Catalog No. ABIN2689388

This Rat Monoclonal antibody specifically detects Transferrin Receptor in FACS. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for Transferrin Receptor antibody (Biotin) (ABIN2689388)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: C2

Product Details anti-Transferrin Receptor Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The C2 antibody reacts with the transferrin receptor CD71, a disulfide-linked homodimer of 95- kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. C2 mAb selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin. Although the isotype of C2 mAb was originally reported to be Rat IgG2a, further investigations have demonstrated that it is Rat IgG1, κ. Two-color analysis of the expression of CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with PE-conjugated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553673) and biotinylated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD71 - Biotin - Clone C2 (also known as C2F2) - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse cell line

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Transferrin Receptor antibody (Biotin) (ABIN2689388)

Lok, Loh: "Regulation of transferrin function and expression: review and update." in: Biological signals and receptors, Vol. 7, Issue 3, pp. 157-78, (1998) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71

Background: Synonyms: Transferrin Receptor

Pathways: Transition Metal Ion Homeostasis