CD90.2 / Thy-1.2 antibody (Biotin)

Catalog No. ABIN2689456

Cited in 16+ publications. The Biotin-conjugated Rat Monoclonal anti-CD90.2 / Thy-1.2 antibody (Clone 3E1) (ABIN2689456) specifically detects CD90.2 / Thy-1.2 in FACS and Sep. The antibody is reactive with Mouse samples.

Quick Overview for CD90.2 / Thy-1.2 antibody (Biotin) (ABIN2689456)

Target: CD90.2 / Thy-1.2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90.2 / Thy-1.2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Separation (Sep)

Clone: 3E1

Product Details anti-CD90.2 / Thy-1.2 Antibody (Biotin)

Characteristics: The 30-H12 clone has been reported to react with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. Thy-1.2 has also been initially reported to be detectable on thymic dendritic cells, but later revealed that the antigen was probably picked up from T-lineage cells. 30-H12 mAb has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that crosslinked 30-H12 antibody induces Ca2+ influx into thymocytes and that co-crosslinking of 30-H12 mAb with antibody to the CD3/TCR complex intensifies thymocyte signal transduction, promotes apoptosis of thymocytes, and inhibits the CD3-mediated proliferative response of mature T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD90.2 - Biotin - Clone 30-H12 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Thymus / Spleen

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD90.2 / Thy-1.2 antibody (Biotin) (ABIN2689456)

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

Borrello, Phipps: "Differential Thy-1 expression by splenic fibroblasts defines functionally distinct subsets." in: Cellular immunology, Vol. 173, Issue 2, pp. 198-206, (1996) (PubMed).

Radrizzani, Carminatti, Pivetta, Idoyaga Vargas: "Developmental regulation of Thy 1.2 rate of synthesis in the mouse cerebellum." in: Journal of neuroscience research, Vol. 42, Issue 2, pp. 220-7, (1996) (PubMed).

Zhong, Donnenberg, Edison, Harrison: "The appearance of Thy-1- donor T cells in the peripheral circulation 3-6 weeks after bone marrow transplantation suggests an extrathymic origin." in: International immunology, Vol. 8, Issue 2, pp. 171-6, (1996) (PubMed).

Wu, Vremec, Ardavin, Winkel, Süss, Georgiou, Maraskovsky, Cook, Shortman: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).

Hueber, Raposo, Pierres, He: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, (1994) (PubMed).

Nakashima, Pu, Hamaguchi, Iwamoto, Rahman, Zhang, Kato, Ohkusu, Katano, Yoshida: "Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3511-20, (1993) (PubMed).

Ikuta, Uchida, Friedman, Weissman: "Lymphocyte development from stem cells." in: Annual review of immunology, Vol. 10, pp. 759-83, (1992) (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).

He, Naquet, Caillol, Pierres: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).

Nakashima, Zhang, Rahman, Yoshida, Isobe, Ding, Iwamoto, Hamaguchi, Ikezawa, Taguchi: "Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 4, pp. 1153-62, (1991) (PubMed).

Tigelaar, Lewis, Bergstresser: "TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue." in: The Journal of investigative dermatology, Vol. 94, Issue 6 Suppl, pp. 58S-63S, (1990) (PubMed).

Kroczek, Gunter, Germain, Shevach: "Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes." in: Nature, Vol. 322, Issue 6075, pp. 181-4, (1986) (PubMed).

Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, (1982) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Target Details for CD90.2 / Thy-1.2

Target: CD90.2 / Thy-1.2

Alternative Name: CD90.2

Background: Synonyms: Thy-1.2