CXCR5 antibody (Biotin)

Catalog No. ABIN2689481

This Rat Monoclonal antibody specifically detects CXCR5 in FACS. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for CXCR5 antibody (Biotin) (ABIN2689481)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR5 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: RF8B2

Product Details anti-CXCR5 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5. Detection of CXCR5 expression on human peripheral lymphocytes by biotinylated RF8B2. Human peripheral blood mononuclear cells were stained with Biotin Rat Anti-Human CXCR5 and FITC Mouse Anti-Human CD3 (Cat. No. 555339, Left Panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412, Right Panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Biotin Rat IgG2b, κ Isotype Control (Cat. No. 555847) and FITC-conjugated mouse IgG2a (Cat. No. 555573) or FITC-conjugated mouse IgG1 (Cat. No. 555748) as isotype controls. The quadrant markers for the bivariate dot plots were set based on the isotype controls.

BD Pharmingen™ Biotin Rat Anti-Human CXCR5 - Biotin - Clone RF8B2 - Isotype Rat IgG2b, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CXCR5

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CXCR5 antibody (Biotin) (ABIN2689481)

Gunn, Ngo, Ansel, Ekland, Cyster, Williams: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).

Legler, Loetscher, Roos, Clark-Lewis, Baggiolini, Moser: "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5." in: The Journal of experimental medicine, Vol. 187, Issue 4, pp. 655-60, (1998) (PubMed).

Förster, Emrich, Kremmer, Lipp: "Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells." in: Blood, Vol. 84, Issue 3, pp. 830-40, (1994) (PubMed).

Kouba, Vanetti, Wang, Schäfer, Höllt: "Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue." in: FEBS letters, Vol. 321, Issue 2-3, pp. 173-8, (1993) (PubMed).

Dobner, Wolf, Emrich, Lipp: "Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma." in: European journal of immunology, Vol. 22, Issue 11, pp. 2795-9, (1992) (PubMed).

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5

Background: Synonyms: CD185, Chemokine C-X-C motif receptor 5, BLR1, MDR-15