EMR4 antibody

Catalog No. ABIN2689502

This Rat Monoclonal antibody specifically detects EMR4 in WB, IP, IHC (fro) and FACS. It exhibits reactivity toward Mouse.

Quick Overview for EMR4 antibody (ABIN2689502)

Target: EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This EMR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: 6F12

Product Details anti-EMR4 Antibody

Characteristics: The 6F12 antibody reacts with a 7-transmembrane-domain protein, which is similar to the F4/80 macrophage antigen of the EGF-TM7 protein family and is encoded by the Emr4 gene. The FIRE protein is expressed on myeloid cells with a denditic cell (DC) developmental potential, including subsets of DC and macrophages in the spleen and lymph nodes, most resident peritoneal macrophages, many peripheral blood monocytes, and a subpopulation of bone-marrow myeloid-cell progenitors. The protein is not detected on peripheral T and B lymphocytes, and it is down-regulated on thioglycollate-elicited peritoneal macrophages and on dendritic cells activated by GM-CSF, IFN-γ, anti-CD40, and LPS. Using soluble biotinylated fusion protein, a FIRE ligand was detected on a mouse IgG+ B lymphoma cell line (A20), but not on myeloid, fibroblast, or T-cell lines, suggesting that the FIRE protein may be involved in immunoregulatory interactions between antigen-presenting cells and B lymphocytes. Three-analysis of the expression of FIRE on bone-marrow leukocytes. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553297, left panels) or purified mAb 6F12 (right panels), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-APC (Cat. No. 554067). Myeloid cells were identified by staining with FITC-conjugated anti-mouse CD11b mAb M1/70 (Cat. No. 557396/553310, upper panels)and dendritic cells were identified with PE-conjugated anti-mouse Cd11c mAb HL3 (Cat. No. 557401/553802, bottom panels). The right panels demonstrate that the hematopoietic precursors which express FIRE protein are CD11b[intermediate]CD11c-. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse F4/80-Like Receptor - Purified - Clone 6F12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CHO cells expressing recombinant FIRE fusion protein

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

Target Details for EMR4

Target: EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))

Alternative Name: F4/80-Like Receptor

Background: Synonyms: FIRE