Fetal Hemoglobin antibody

Catalog No. ABIN2689504

This anti-Fetal Hemoglobin antibody is a Mouse Monoclonal antibody detecting Fetal Hemoglobin in FACS. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Fetal Hemoglobin antibody (ABIN2689504)

Target: Fetal Hemoglobin (HbF)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fetal Hemoglobin antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 2D12

Product Details anti-Fetal Hemoglobin Antibody

Brand: BD Pharmingen™

Characteristics: Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1 % of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of erythrocytes obtained from cord blood analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human Fetal Hemoglobin - Purified - Clone 2D12 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Fetal Hemoglobin antibody (ABIN2689504)

Campbell, Ware, Mason: "Detection of hemoglobin variants in erythrocytes by flow cytometry." in: Cytometry, Vol. 35, Issue 3, pp. 242-8, (1999) (PubMed).

Horiuchi, Osterhout, Kamma, Bekoe, Hirokawa: "Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry." in: Cytometry, Vol. 20, Issue 3, pp. 261-7, (1995) (PubMed).

Thorpe, Thein, Sampietro, Craig, Mahon, Huehns: "Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis." in: British journal of haematology, Vol. 87, Issue 1, pp. 125-32, (1994) (PubMed).

Target Details for Fetal Hemoglobin

Target: Fetal Hemoglobin (HbF)

Alternative Name: Fetal Hemoglobin