Gammadelta T Lymphocytes antibody

Catalog No. ABIN2689515

There are 3+ publications for this product available. The Rat Monoclonal anti-Gammadelta T Lymphocytes antibody is suitable to detect Gammadelta T Lymphocytes in samples from Pig. It has been validated for CyTox, IHC (fro), FACS and IP.

Quick Overview for Gammadelta T Lymphocytes antibody (ABIN2689515)

Target: Gammadelta T Lymphocytes

Reactivity: Pig

Host: Rat

Clonality: Monoclonal

Application: Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MAC320

Product Details

Characteristics: The MAC320 antibody reacts with the 270- and 280- kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes. Two-color analysis of pig peripheral blood γδ T lymphocytes. Pig whole blood was stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no.553927, left panel) or purified mAb MAC320 (center and right anels), followed by biotinylated mouse anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553894, all panels) then Streptavidin-PE (Cat. no.554061, all panels). In the right panel, anti-pig γδ TCR mAb PGBL22A (VMRD, Pullman, WA) was added in a fourth staining step, followed by FITC-conjugated rat anti-mouse IgG1 mAb A85-1. Erythrocytes were lysed (PharM Lyse™, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815), and lymphocytes were gated according to their ligh-scatter profile. Flow cytometry was performed on a FACSCalibur™ . The right panel demonstrates that MAC320+ cells express the gd TCR and that there are MAC320+ and MAC320- subpopulations of γδ TCR-bearing T lymphocytes in the blood.

BD Pharmingen™ Purified Rat Anti-Pig γδ T Lymphocytes - Purified - Clone MAC320 - Isotype Rat IgG2a, κ - Reactivity Pig - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Null T cells purified from the blood of young pigs

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Davis, Zuckermann, Hamilton, Barbosa, Saalmüller, Binns, Licence: "Analysis of monoclonal antibodies that recognize gamma delta T/null cells." in: Veterinary immunology and immunopathology, Vol. 60, Issue 3-4, pp. 305-16, (1998) (PubMed).

Yang, Parkhouse: "Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues." in: Immunology, Vol. 89, Issue 1, pp. 76-83, (1996) (PubMed).

Binns: "The Null/gamma delta TCR+ T cell family in the pig." in: Veterinary immunology and immunopathology, Vol. 43, Issue 1-3, pp. 69-77, (1995) (PubMed).

Target Details

Target: Gammadelta T Lymphocytes