MHC Class I (H-2Kb/H-2Db) antibody (Biotin)

Catalog No. ABIN2689543

This anti- antibody is a Mouse Monoclonal antibody detecting in FACS. Suitable for Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for MHC Class I (H-2Kb/H-2Db) antibody (Biotin) (ABIN2689543)

Target: MHC Class I (H-2Kb/H-2Db)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: 28-8

Product Details

Brand: BD Pharmingen™

Characteristics: The 28-8-6 antibody reacts with the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (e.g., f, k, p, q, r, s) has not been observed.

BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]/H-2D[b] - Biotin - Clone 28-8-6 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H.SW mouse splenocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).

Evans, Margulies, Shykind, Seidman, Ozato: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).

Target Details

Target: MHC Class I (H-2Kb/H-2Db)

Alternative Name: H-2K[b]/H-2D[b]