MHC II (I-A/I-E) antibody (Biotin)

Catalog No. ABIN2689578

The Rat Monoclonal anti-MHC II (I-A/I-E) antibody is suitable to detect MHC II (I-A/I-E) in samples from Mouse. It has been validated for FACS and IHC (fro).

Quick Overview for MHC II (I-A/I-E) antibody (Biotin) (ABIN2689578)

Target: MHC II (I-A/I-E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC II (I-A/I-E) antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2G9

Product Details anti-MHC II (I-A/I-E) Antibody (Biotin)

Characteristics: The 2G9 antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 (Cat. No. 556999, for purified mAb) monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

BD Pharmingen™ Biotin Rat Anti-Mouse I-A/I-E - Biotin - Clone 2G9 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse epidermal Langerhans cells

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Target Details for MHC II (I-A/I-E)

Target: MHC II (I-A/I-E)

Alternative Name: I-A/I-E